This research article presents a novel two-photon microscopy protocol for real-time visualization of NAD+ metabolism in brain endothelial cells in living mice. The study demonstrates technical methods to monitor how oral NMN (nicotinamide mononucleotide) delivery affects cerebrovascular NAD+ biosensor fluorescence, providing mechanistic evidence of NMN’s bioavailability and metabolic impact at the blood-brain barrier. This validates in vivo NAD+ pathway engagement as a measurable biomarker for oral peptide and nutrient supplements targeting neurometabolism.
For your business
Mouse imaging confirms oral NMN reaches brain endothelium and shifts NAD+ metabolism at the blood-brain barrier, supporting mechanistic rationale for cerebrovascular benefit.